Generator

Part:BBa_K199135:Design

Designed by: Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2010-03-30)


Optimized positive feedback RFP generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 462
    Illegal AgeI site found at 1405
    Illegal AgeI site found at 1517
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 135


Design Notes

In pSB1A2. LuxI has 7 amino acids more than the V. fischeri LuxI (C0161) on the amino terminus.


Source

From the BioBrick Parts registry, and de novo synthesis of optimized LuxI.

References